Composite

Part:BBa_K2933198:Design

Designed by: Jian Dong   Group: iGEM19_TJUSLS_China   (2019-09-16)


T7 promoter+RBS b+linker h+His+Linker a+Sumo+Linker b+ARL-1+T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
    Illegal PstI site found at 916
    Illegal PstI site found at 1030
    Illegal PstI site found at 1081
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 390
    Illegal NheI site found at 167
    Illegal NheI site found at 1346
    Illegal PstI site found at 916
    Illegal PstI site found at 1030
    Illegal PstI site found at 1081
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 390
    Illegal BglII site found at 279
    Illegal BamHI site found at 478
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
    Illegal PstI site found at 916
    Illegal PstI site found at 1030
    Illegal PstI site found at 1081
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
    Illegal PstI site found at 916
    Illegal PstI site found at 1030
    Illegal PstI site found at 1081
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

NCBI

References